Isolation and application of bacteriophages alone or in combination with nisin against planktonic and biofilm cells of Staphylococcus aureus

Abstract

Staphylococcus aureus is a notorious foodborne pathogen since it has ability to produce variety of toxins including heat-stable enterotoxin, form biofilm, and acquire resistance to antibiotics. Biocontrol of foodborne pathogens by lytic bacteriophages garners increasing interest from both researchers and food industry. In the present study, 29 phages against S. aureus were successfully isolated from chicken, pork, and fish. Characterization of the isolates revealed that phage SA46-CTH2 belonging to Podoviridae family had a number of features suitable for food industry applications such as wide host range, short latent period, large burst size, high stress tolerance, and a genome free of virulence genes. Furthermore, phage SA46-CTH2 alone or in combination with nisin exhibited great efficacy in reducing planktonic and biofilm cells of S. aureus at various conditions tested. The combination of phage SA46-CTH2 and nisin was also found to be able to inhibit the regrowth of S. aureus at both 37 and 24 °C.

Hoang Minh Duc

FVM- Vietnam National University of Agriculture

Keywords: Bacteriophages, S. aureus, Biofilm, Antibiotic Resistance Link

40 thoughts on “Application of a real-time PCR assay for the detection of Ascaris suum DNA in the liver of experimentally infected chickens

  1. orgone says:

    Hello! This is my 1st comment here so I just wanted to give a quick shout out and say I truly enjoy reading through your blog posts. Can you recommend any other blogs/websites/forums that deal with the same topics? Thanks a ton!

  2. orgone says:

    ) Znovu ho navštívím, protože jsem si ho poznamenal. Peníze a svoboda je nejlepší způsob, jak se změnit, ať jste bohatí a

  3. soodne naiste aluspesu says:

    Esta página tem definitivamente toda a informação que eu queria sobre este assunto e não sabia a quem perguntar. Este é o meu primeiro comentário aqui, então eu só queria dar um rápido

  4. sale of used panties says:

    det. Denne side har bestemt alle de oplysninger, jeg ønskede om dette emne, og vidste ikke, hvem jeg skulle spørge. Dette er min 1. kommentar her, så jeg ville bare give en hurtig

  5. orgone says:

    nenarazili jste někdy na problémy s plagorismem nebo porušováním autorských práv? Moje webové stránky mají spoustu unikátního obsahu, který jsem vytvořil.

  6. gebrauchte Damenschuhe says:

    Esta página tem definitivamente toda a informação que eu queria sobre este assunto e não sabia a quem perguntar. Este é o meu primeiro comentário aqui, então eu só queria dar um rápido

  7. camgirl says:

    Does your blog have a contact page? I’m having problems locating it but, I’d like to send you an email. I’ve got some ideas for your blog you might be interested in hearing. Either way, great website and I look forward to seeing it improve over time.

Leave a Reply

Your email address will not be published. Required fields are marked *