Publications, Science and Technology

Application of a real-time PCR assay for the detection of Ascaris suum DNA in the liver of experimentally infected chickens

Ngày đăng: by Khoa Thú y

Abstract

This study aimed to evaluate the sampling method for the detection of Ascaris suum larval DNA in chicken livers using real-time PCR. Chickens were inoculated with A. suum eggs of a single dose (Group A) or repeatedly low doses (Group B). White spots (WSs) were continuously observed on liver from day 3 after the last infection in Group B and day 14 in Group A. In Group A, larval DNA was detected in WS lesions (78.6%) at a significantly higher rate than in the remaining tissue samples (31.3%). In conclusion, applying WS lesions to the assay improved the detection rate of A. suum DNA in chicken livers, especially in the case of a single infection.

Yen Thi Hoang Nguyen

FVM- Vietnam National University of Agriculture

Keywords: Ascaris suum; chicken liver; real-time PCR assay; white spot lesion. Link

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