Publications

First contribution to water buffalo (Bubalus bubalis) type-I/III interferons – induced Mx1 detection and anti-viral activity

Ngày đăng: by Khoa Thú y
Introduction Myxovirus resistance protein 1 (Mx1) is a type I/III interferons-induced protein conferring the hosts an antiviral property against several viruses. The protein is also found to be a potential biomarker for early detection of viral infections in many vertebrates. In humans, MxA and MxB proteins have been studied intensively for decades and it has been found that the normal concentration of MxA protein in peripheral blood of healthy people is less than 50 ng/mL, that it is induced very shortly (1-2 hours) after exposure to viral infections and that it displays a half-life of 2-3 days. Human MxA protein in peripheral blood of patients has been shown to be clinically sensitive and specific biomarker for viral infections. The blood MxA concentration is increased specifically by infections by Rhinoviruses, respiratory syncytial viruses, parainfluenza viruses, influenza viruses, coronaviruses, and human metapneumoviruses. In human medicine, MxA protein has been used successfully as a specific biomarker for distinguishing viral to bacterial infections, providing good guidance for diagnostic and prescription of antibiotics. Several studies have been conducted on Mx1 protein of many livestock including pig, dog, horse and cow. However, there is no research conducted on the proteins Mx of water buffalo, even if this valuable ruminant, which is vulnerable to several dangerous viral diseases, has been increasingly economically important in Asia. Objectives In an attempt to develop a new, effective, cheap and rapid diagnostic tool, which can be used effectively for the control of viral diseases by screening for elimination of domestic water buffalo carrying viruses including asymptomatic individuals, in the present study, we initially succeeded in production and characterization of recombinant buffalo (Bubalus bubalis) Mx1 protein (bbMx1) and a series of monoclonal antibodies specific to the bbMx1 protein. Methodology In order to produce recombinant bbMx1 protein, two vectors were synthesized for expressing a reference buffalo Mx1 either in Escherichia coli or in mammalian cells. In both cases, the recombinant water buffalo Mx1 was intendedly fused to a N-term functional group with either a poly-histidine tract or a V5 epitope. In the latter, the V5-bbMx1 protein was further fused to a N-term bipartite group consisting of the fluorescent marker protein RFP followed by the spontaneously cleavable peptide 2A. The recombinant bbMx1 was expressed successfully in both E. coli and mammalian cells by transient transfection using Lipofectamin 3,000 reagent. SDS-PAGE analysis of cells lysate from transfected cell population and poly (I:C)-incubated buffalo cells illustrated of bbMx1 protein with molecular weight of almost 75kDa. The hybridomas expressing antibodies specific to bbMx1 were established by fusion of SP2/0myeloma cells with spleenocytes derived from female BALB/c mice, which had been hyper-immunized with the recombinant V5-bbMx1 protein. A set of 10 mouse hybridomas producing mAbs to bbMx1 was obtained. The 10 corresponding mAbs were further characterized using indirect ELISA, Western blot analysis and immunocytofluorescent staining. Conclusions Eight mAbs, designated 11C7, RD5, NF3, 9D1, FD4, PE6, 11A7 and 10F5, displayed binding abilities and specificity to recombinant bbMx1 in the three formats. Moreover, combining NF3 (for capture) with RD5 (for detection), 9D1 with RD5, FD4 with 11C7 or PE6 with 11C7 generated a strong signal in a prototype sandwich-ELISA. In addtion, mAbs RD5 and 11A7 also illustrated to bind specifically to wildtype bbMx1 protein, which was expressed by bubaline poly-I/C-stimulated mesenchymal stem cells in immunoblotting format. Interestingly, it was also observed that there were cross reactions between mAbs and recombinant proteins among species. NF3, which was a specifically designed mAb targeting bovine Mx1 protein, bound specifically to buffalo Mx1 protein too. Meanwhile, 10B4, 11A7, 11C7 and 9D1, the mAbs specific to buffalo Mx1 protein also showed binding abilities to recombinant porcine Mx1 protein in western blot tests. The results suggest that the mAbs developed and characterized here provide an excellent starting point for developing diagnostic tools aimed at detecting viral infections in the water buffalo, whatever using immunoblotting, immunocytostaining, flow cytometry or sandwich-ELISA. Furthermore, type I/III interferons provide powerful and universal innate intracellular defense mechanisms against viruses. Among the antiviral effectors induced, Mx proteins of some species appear as key components of defense against influenza A viruses. It is expected that such an antiviral protein must display a platform dedicated to the recognition of said viruses. In my thesis, the anti-influenza activity of bbMx1 and other four distinct mammalian Mx1 proteins was measured by comparing the number of viral nucleoprotein-positive cells 7 hours after infection in a sample of 100,000 cells expected to contain both Mx1-positive and Mx1-negative cell subpopulations. The systematic depletion (p < 0.001) of virus nucleoprotein-positive cells among equine, bubaline, porcine and bovine Mx1-expressing cell populations compared to Mx-negative cells suggests a strong anti-influenza A activity. Looking for common anti-influenza signature elements in the sequence of these Mx proteins, we found that an aromatic residue at positions 561 or 562 in the L4 loop seems critical for the anti-influenza function and/or specificity of mammalian Mx1. Từ khóa: Bubalus bubalis, myxovirus resistance protein_1, water bufalo, monocnonal antibodies, water bufalo Link:  https://orbi.uliege.be/handle/2268/242357

PHAI DAM VAN

Vietnam National University of Agriculture

102 thoughts on “Triple gene editing in porcine embryos using electroporation alone or in combination with microinjection”

  36. https://menbehealth.wordpress.com/ says:

    Unquestionably consider that which you stated.
    Your favourite justificstion appeared to be at the
    web the easiest thing to be aware of. I say to you,
    I definitely get irked att the same time as people consider wories that they jut don’t realize about.
    You controlled to hit the nail upon tthe top and defined out the whole
    thing without having side-effects , folks can take
    a signal. Will probably be back to get more. Thank you https://menbehealth.wordpress.com/

  41. Code of destiny says:

    I am really impressed with your writing talents and also with the structure for your blog. Is that this a paid theme or did you customize it your self? Anyway keep up the excellent high quality writing, it is uncommon to peer a great blog like this one these days!

  44. metamask login says:

    MetaMask Download is beginner-friendly. The setup is straightforward, and the security features provide peace of mind for new users.

  47. 1wiN says:

    Официальный Telegram канал 1win Casinо. Казинo и ставки от 1вин. Фриспины, актуальное зеркало официального сайта 1 win. Регистрируйся в ван вин, соверши вход в один вин, получай бонус используя промокод и начните играть на реальные деньги.
    https://t.me/s/Official_1win_kanal/501

  53. lindsey haley says:

    Thanks for making it so relatable. Watch onua tv morning show today — live shows, news, entertainment, and morning programs from Ghana. Enjoy interviews, highlights, and replays with stable, high‑quality streaming. Including today’s schedule, morning show and interviews.

  63. Browse Heart Rate Monitors says:

    One thing I’d like to say is the fact before buying more laptop memory, consider the machine in to which it could well be installed. If the machine is usually running Windows XP, for instance, a memory ceiling is 3.25GB. Installing in excess of this would just constitute some sort of waste. Make sure one’s motherboard can handle the particular upgrade volume, as well. Thanks for your blog post.

  64. Adjustable Dumbbells & Weights says:

    Hmm is anyone else experiencing problems with the images on this blog loading? I’m trying to figure out if its a problem on my end or if it’s the blog. Any feed-back would be greatly appreciated.

  65. sexvip2025xxx.lat says:

    Thanks , I have recently been looking for information about this topic for ages and yours is the greatest I have discovered so far. But, what about the bottom line? Are you sure about the source?

  66. sexprobet.lat says:

    I really appreciate this post. I have been looking all over for this! Thank goodness I found it on Bing. You’ve made my day! Thank you again

  67. Full-Body Fitness Coaching says:

    Usually I do not read post on blogs, but I wish to say that this write-up very compelled me to check out and do so! Your writing taste has been surprised me. Thanks, quite great post.

  69. AYUTOGEL says:

    A powerful share, I simply given this onto a colleague who was doing a little analysis on this. And he in actual fact bought me breakfast as a result of I discovered it for him.. smile. So let me reword that: Thnx for the treat! However yeah Thnkx for spending the time to discuss this, I feel strongly about it and love studying more on this topic. If possible, as you change into experience, would you mind updating your blog with extra details? It’s highly helpful for me. Huge thumb up for this weblog submit!

  70. togelup says:

    F*ckin? remarkable things here. I?m very glad to see your post. Thanks a lot and i’m looking forward to contact you. Will you please drop me a e-mail?

  71. State Farm Insurance Agent says:

    hello there and thank you for your info ? I have definitely picked up something new from right here. I did however expertise several technical points using this website, as I experienced to reload the site a lot of times previous to I could get it to load properly. I had been wondering if your web hosting is OK? Not that I’m complaining, but sluggish loading instances times will sometimes affect your placement in google and can damage your quality score if advertising and marketing with Adwords. Anyway I?m adding this RSS to my e-mail and can look out for much more of your respective fascinating content. Make sure you update this again soon..

  72. insurance agency Perrysburg says:

    This is really interesting, You are a very skilled blogger. I have joined your rss feed and look forward to seeking more of your great post. Also, I’ve shared your website in my social networks!

  73. insurance agency near me says:

    I do like the manner in which you have presented this situation and it does indeed provide us some fodder for thought. On the other hand, because of just what I have personally seen, I basically trust as the responses pile on that folks remain on point and in no way get started on a soap box of some other news du jour. Yet, thank you for this fantastic piece and while I do not really go along with the idea in totality, I regard your viewpoint.

  74. insurance agency Perrysburg says:

    Definitely imagine that which you stated. Your favorite reason appeared to be at the net the simplest factor to bear in mind of. I say to you, I definitely get irked even as other folks think about issues that they plainly don’t realize about. You controlled to hit the nail upon the top as neatly as defined out the whole thing without having side effect , other people can take a signal. Will likely be back to get more. Thank you

  75. good dry food for cats says:

    Good post made here. One thing I would like to say is that often most professional career fields consider the Bachelors Degree as the entry level standard for an online education. When Associate Qualifications are a great way to start, completing your current Bachelors uncovers many doors to various jobs, there are numerous online Bachelor Course Programs available through institutions like The University of Phoenix, Intercontinental University Online and Kaplan. Another thing is that many brick and mortar institutions provide Online versions of their college diplomas but often for a drastically higher cost than the companies that specialize in online higher education degree plans.

  76. insurance agency says:

    Thanks for the diverse tips provided on this website. I have realized that many insurance agencies offer prospects generous discount rates if they choose to insure a few cars together. A significant variety of households have several cars these days, in particular those with elderly teenage youngsters still located at home, along with the savings for policies can easily soon begin. So it is a good idea to look for a great deal.

  78. insurance agency near me says:

    Its like you read my mind! You seem to know a lot about this, like you wrote the book in it or something. I think that you could do with a few pics to drive the message home a bit, but other than that, this is magnificent blog. An excellent read. I will definitely be back.

  79. insurance agency near me says:

    hi!,I like your writing very much! share we communicate more about your post on AOL? I need a specialist on this area to solve my problem. May be that’s you! Looking forward to see you.

  80. Alex Wakefield - State Farm Insurance Agent says:

    Today, I went to the beachfront with my kids. I found a sea shell and gave it to my 4 year old daughter and said “You can hear the ocean if you put this to your ear.” She put the shell to her ear and screamed. There was a hermit crab inside and it pinched her ear. She never wants to go back! LoL I know this is completely off topic but I had to tell someone!

  81. custom tron vanity address says:

    The following time I learn a blog, I hope that it doesnt disappoint me as a lot as this one. I mean, I know it was my option to learn, but I actually thought youd have something interesting to say. All I hear is a bunch of whining about one thing that you might fix when you werent too busy on the lookout for attention.

  82. Devon Mack says:

    Great post here. One thing I would like to say is most professional domains consider the Bachelors Degree like thejust like the entry level requirement for an online diploma. Though Associate Degrees are a great way to get started on, completing your Bachelors reveals many doors to various occupations, there are numerous on-line Bachelor Diploma Programs available via institutions like The University of Phoenix, Intercontinental University Online and Kaplan. Another issue is that many brick and mortar institutions provide Online variants of their diplomas but typically for a significantly higher cost than the companies that specialize in online qualification programs.

  83. trc20 token generator says:

    Excellent post but I was wanting to know if you could write a litte more on this topic? I’d be very grateful if you could elaborate a little bit more. Bless you!

  84. insurance agency says:

    Yet another issue is really that video gaming has become one of the all-time main forms of excitement for people of all ages. Kids enjoy video games, plus adults do, too. The particular XBox 360 has become the favorite video games systems for people who love to have hundreds of video games available to them, along with who like to experiment with live with other individuals all over the world. Many thanks for sharing your ideas.

  85. insurance agency says:

    Good day! Do you know if they make any plugins to assist with Search Engine Optimization? I’m trying to get my blog to rank for some targeted keywords but I’m not seeing very good results. If you know of any please share. Many thanks!

  86. Colin Fane - State Farm Insurance Agent says:

    Its like you read my mind! You seem to know so much about this, like you wrote the book in it or something. I think that you could do with a few pics to drive the message home a bit, but instead of that, this is magnificent blog. An excellent read. I will certainly be back.

  87. Jared Mula - State Farm Insurance Agent says:

    One thing I would like to comment on is that fat burning plan fast can be achieved by the perfect diet and exercise. Someone’s size not merely affects the look, but also the quality of life. Self-esteem, depressive disorders, health risks, along with physical ability are afflicted in excess weight. It is possible to make everything right whilst still having a gain. If this happens, a problem may be the reason. While too much food instead of enough exercising are usually accountable, common health concerns and trusted prescriptions can greatly add to size. Many thanks for your post in this article.

  89. Cole Green - State Farm Insurance Agent says:

    Hmm it appears like your website ate my first comment (it was super long) so I guess I’ll just sum it up what I had written and say, I’m thoroughly enjoying your blog. I too am an aspiring blog blogger but I’m still new to everything. Do you have any helpful hints for inexperienced blog writers? I’d genuinely appreciate it.

  90. insurance agency near me says:

    Undeniably believe that which you said. Your favorite reason appeared to be on the net the easiest thing to be aware of. I say to you, I definitely get irked while people think about worries that they just don’t know about. You managed to hit the nail upon the top as well as defined out the whole thing without having side-effects , people could take a signal. Will probably be back to get more. Thanks

  91. Cole Green - State Farm Insurance Agent says:

    I liked up to you will receive carried out proper here. The sketch is attractive, your authored subject matter stylish. nevertheless, you command get bought an impatience over that you would like be turning in the following. in poor health undoubtedly come more until now once more as exactly the same just about a lot incessantly inside case you defend this increase.

  92. games with private servers says:

    I know this if off topic but I’m looking into starting my own blog and was curious what all is required to get setup? I’m assuming having a blog like yours would cost a pretty penny? I’m not very internet savvy so I’m not 100 certain. Any tips or advice would be greatly appreciated. Cheers

  93. pserver list says:

    Good day! This post could not be written any better! Reading this post reminds me of my good old room mate! He always kept talking about this. I will forward this article to him. Pretty sure he will have a good read. Thank you for sharing!

  94. Andrew Smith - State Farm Insurance Agent says:

    Excellent goods from you, man. I’ve understand your stuff previous to and you are just too wonderful. I actually like what you have acquired here, really like what you are saying and the way in which you say it. You make it enjoyable and you still take care of to keep it wise. I can not wait to read far more from you. This is really a great web site.

  96. Anita A Murray says:

    Heya i am for the first time here. I found this board and I find It really useful & it helped me out much. I hope to give something back and help others like you aided me.

  97. insurance agency says:

    Thanks for the suggestions you discuss through this website. In addition, a lot of young women exactly who become pregnant don’t even aim to get health care insurance because they worry they wouldn’t qualify. Although a few states now require that insurers give coverage regardless of pre-existing conditions. Fees on these guaranteed plans are usually larger, but when thinking about the high cost of medical care it may be a new safer approach to take to protect your current financial future.

  98. Adam Garcia - State Farm Insurance Agent says:

    Thanks alot : ) for your post. I’d like to comment that the cost of car insurance differs a lot from one coverage to another, for the reason that there are so many different issues which play a role in the overall cost. For example, the model and make of the car will have an enormous bearing on the fee. A reliable older family car or truck will have an inexpensive premium than just a flashy fancy car.

  101. intervento perdita acqua urgente says:

    I would like to thank you for the efforts you have put in writing this blog. I’m hoping the same high-grade website post from you in the upcoming also. Actually your creative writing skills has inspired me to get my own blog now. Really the blogging is spreading its wings quickly. Your write up is a great example of it.

  102. Adam Garcia says:

    Someone essentially help to make seriously articles I would state. This is the very first time I frequented your web page and thus far? I surprised with the research you made to make this particular publish incredible. Great job!

Leave a Reply

Your email address will not be published. Required fields are marked *