Molecular epidemiology of canine parvovirus type 2 in Vietnam from November 2016 to February 2018

Abstract

Background

Canine parvovirus type 2 (CPV-2) was first identified in the late 1970s; it causes intestinal hemorrhage with severe bloody diarrhea in kennels and dog shelters worldwide. Since its emergence, CPV-2 has been replaced with new genetic variants (CPV-2a, CPV-2b, and CPV-2c). Currently, information about the genotype prevalence of CPV-2 in Vietnam is limited. In the present study, we investigated the genotype prevalence and distribution of CPV-2 in the three regions of Vietnam.

Methods

Rectal swabs were collected from 260 dogs with suspected CPV-2 infection from northern, central, and southern Vietnam from November 2016 to February 2018. All samples were identified as parvovirus positive by real-time PCR, and further genotyping was performed using a SimpleProbe® real-time PCR assay.

Results

Of the 260 Vietnamese CPV-2 isolates, 6 isolates (2.31%) were identified as CPV-2a, 251 isolates (96.54%) were identified as CPV-2c and 3 isolates (1.15%) were untypable using the SimpleProbe® real-time PCR assay. In northern Vietnam, the percentages of CPV-2a and CPV-2c were 2.97% (3/101) and 97.3% (98/101), respectively. In central Vietnam, the percentages of CPV-2a and CPV-2c were 1.11% (1/90) and 98.89% (89/90), respectively. In southern Vietnam, the percentages of CPV-2a and CPV-2c were 3.03% (2/66) and 96.97% (64/66), respectively. CPV-2b was not observed in this study. The VP2 genes of CPV-2c in Vietnam are more genetically similar to those of CPV-2c strains in China and Taiwan than to those of prototype CPV-2c strains (FJ222821) or the first Vietnamese CPV-2c (AB120727).

Conclusion

The present study provides evidence that CPV-2c is the most prevalent variant in Vietnam. Phylogenetic analysis demonstrated that the recent Vietnamese CPV-2c isolates share a common evolutionary origin with Asian CPV-2c strains.

Minh Hoang

FVM- Vietnam National University of Agriculture

39 thoughts on “Triple gene editing in porcine embryos using electroporation alone or in combination with microinjection

  1. https://menbehealth.wordpress.com/ says:

    Unquestionably consider that which you stated.
    Your favourite justificstion appeared to be at the
    web the easiest thing to be aware of. I say to you,
    I definitely get irked att the same time as people consider wories that they jut don’t realize about.
    You controlled to hit the nail upon tthe top and defined out the whole
    thing without having side-effects , folks can take
    a signal. Will probably be back to get more. Thank you https://menbehealth.wordpress.com/

Leave a Reply

Your email address will not be published. Required fields are marked *