Establishment of an In Vitro Model of Persistent Chicken Anemia Virus Infection

Persistent infection of chicken anemia virus (CAV) in chickens has been suspected to result in immunosuppression and exogenous virus contamination within vaccine production. However, no direct evidence for persistent CAV infection has thus far been obtained. In this study, we aimed to establish an in vitro model of persistent CAV infection. CAV-infected MDCC-MSB1 (MSB1) cells, a Marek’s disease virus-transformed continuous cell line, were cultured in the presence of both CAV and CAV neutralizing antibody (NA). Cell viability, expression of viral antigens, viral DNA, and recovery of CAV were examined by acridine orange/propidium iodide staining, immunofluorescence measurement, real-time PCR, and viral isolation, respectively. The results indicated that CAV was maintained and possibly replicated in CAV-infected cells cultured in the presence of NA, without affecting host cell viability. It was also shown that persistently infectious CAV induced cell death again after removing NA. The persistent infection of CAV in MSB1 cells was not related to viral gene mutation. In summary, we have herein established a novel model of persistent CAV infection in MSB1 cells cultured in the presence of NA.
Dong Van Hieu

FVM- Vietnam National University of Agriculture

79 thoughts on “Application of a real-time PCR assay for the detection of Ascaris suum DNA in the liver of experimentally infected chickens

  1. orgone says:

    Hello! This is my 1st comment here so I just wanted to give a quick shout out and say I truly enjoy reading through your blog posts. Can you recommend any other blogs/websites/forums that deal with the same topics? Thanks a ton!

  2. orgone says:

    ) Znovu ho navštívím, protože jsem si ho poznamenal. Peníze a svoboda je nejlepší způsob, jak se změnit, ať jste bohatí a

  3. soodne naiste aluspesu says:

    Esta página tem definitivamente toda a informação que eu queria sobre este assunto e não sabia a quem perguntar. Este é o meu primeiro comentário aqui, então eu só queria dar um rápido

  4. sale of used panties says:

    det. Denne side har bestemt alle de oplysninger, jeg ønskede om dette emne, og vidste ikke, hvem jeg skulle spørge. Dette er min 1. kommentar her, så jeg ville bare give en hurtig

  5. orgone says:

    nenarazili jste někdy na problémy s plagorismem nebo porušováním autorských práv? Moje webové stránky mají spoustu unikátního obsahu, který jsem vytvořil.

  6. gebrauchte Damenschuhe says:

    Esta página tem definitivamente toda a informação que eu queria sobre este assunto e não sabia a quem perguntar. Este é o meu primeiro comentário aqui, então eu só queria dar um rápido

  7. camgirl says:

    Does your blog have a contact page? I’m having problems locating it but, I’d like to send you an email. I’ve got some ideas for your blog you might be interested in hearing. Either way, great website and I look forward to seeing it improve over time.

  8. 蕾丝内衣 says:

    This is really interesting, You are a very skilled blogger. I’ve joined your rss feed and look forward to seeking more of your excellent post. Also, I’ve shared your site in my social networks!

  9. kasutatud aluspesu müük says:

    |Tato stránka má rozhodně všechny informace, které jsem o tomto tématu chtěl a nevěděl jsem, koho se zeptat.|Dobrý den! Tohle je můj 1. komentář tady, takže jsem chtěl jen dát rychlý

  10. fare l'amore says:

    Hmm it seems like your website ate my first comment (it was extremely long) so I guess I’ll just sum it up what I submitted and say, I’m thoroughly enjoying your blog. I as well am an aspiring blog blogger but I’m still new to everything. Do you have any tips for rookie blog writers? I’d really appreciate it.

  11. ondergoed says:

    har også bogmærket dig for at se på nye ting på din blog Hej! Har du noget imod, hvis jeg deler din blog med min facebook

  12. pensierino per un'amica says:

    at web, except I know I am getting familiarity all the time by reading thes pleasant posts.|Fantastic post. I will also be handling some of these problems.|Hello, I think this is a great blog. I happened onto it;) I have bookmarked it and will check it out again. The best way to change is via wealth and independence. May you prosper and never stop mentoring others.|I was overjoyed to find this website. I must express my gratitude for your time because this was an amazing read! I thoroughly enjoyed reading it, and I’ve bookmarked your blog so I can check out fresh content in the future.|Hi there! If I shared your blog with my Facebook group, would that be okay? I believe there are a lot of people who would truly value your article.|منشور رائع. سأتعامل مع بعض هذه|

  13. mutandine donna per incontinenza says:

    Hello! Someone in my Facebook group shared this site with us so I came to give it a look. I’m definitely enjoying the information. I’m book-marking and will be tweeting this to my followers! Fantastic blog and excellent style and design.

  14. 回收 二手内衣裤 says:

    Wonderful blog! I found it while searching on Yahoo News. Do you have any suggestions on how to get listed in Yahoo News? I’ve been trying for a while but I never seem to get there! Thank you

  15. https://menbehealth.wordpress.com/ says:

    Unquestionaly consider that which you stated. Your favourite justification appeared to
    be at the web the easiest thing to be aware of. I say to you, I definitely
    get irked at the same time as people consider worries
    that thdy just don’trealize about. You controlled to hit the nail
    upon the top and defined out the whole thing without having side-effects , folks can take a signal.
    Will probably be back to get more. Thank you https://menbehealth.wordpress.com/

  16. idea regalo uomo says:

    Hitclub là một trong những cổng game đổi thưởng nổi bật nhất hiện nay, nơi bạn không chỉ có thể tận hưởng những giây phút giải trí mà còn có cơ hội nhận về những phần thưởng hấp dẫn. Với nhiều trò chơi phong phú và giao diện thân thiện, cổng game này thực sự đã chinh phục được lòng tin của đông đảo người chơi.

Leave a Reply

Your email address will not be published. Required fields are marked *