This study evaluated the sensitivity of biotinyl-tyramide-based in situ hybridization (TISH) method by comparison with chromogenic in situ hybridization (CISH) and immunohistochemical staining (IHC) methods. This study also determined the effect of fixative and fixation time on the detection of Porcine reproductive and respiratory syndrome virus (PRRSV) in paraffin-embedded tissues. Lung samples were fixed in 4% paraformaldehyde (PFA) or 10% neutral buffered formalin (NBF) for various times before paraffin embedding. Of 30 paraffin-embedded lung samples, fixed for 1 day in 4% PFA or 10% NBF, 18 (60%) were positive for PRRSV by nested reverse transcription polymerase chain reaction (nRT-PCR). All 18 lung samples (100%) also were positive for PRRSV by TISH, but only 10 of these 18 specimens (56%) were positive for PRRSV by IHC and CISH. We demonstrated that TISH can detect PRRSV RNA in paraffin-embedded tissues after up to 90 days of fixation. PRRSV nucleic acids and antigens were better preserved in 4% PFA than in 10% NBF. Compared with CISH and IHC testing methods, TISH appeared to be more sensitive for the detection of PRRSV in paraffin-embedded tissues.
Keywords: In situ hybridization; Porcine reproductive and respiratory syndrome virus; lung.
FVM, Vietnam National University of Agriculture
Miyazaki University, Japan
Link
- Home /
- Publications, Science and Technology
- / Application of a real-time PCR assay for the detection of Ascaris suum DNA in the liver of experimentally infected chickens
Tiếng Việt
I like it when people get together and share thoughts. Great blog, keep it up!
Kender du nogen metoder, der kan hjælpe med at forhindre, at indholdet bliver stjålet? Det ville jeg sætte stor pris på.
buď vytvořil sám, nebo zadal externí firmě, ale vypadá to.
Hello! This is my 1st comment here so I just wanted to give a quick shout out and say I truly enjoy reading through your blog posts. Can you recommend any other blogs/websites/forums that deal with the same topics? Thanks a ton!
) Znovu ho navštívím, protože jsem si ho poznamenal. Peníze a svoboda je nejlepší způsob, jak se změnit, ať jste bohatí a
reading this weblog’s post to be updated daily.
for the reason that here every material is quality based
O conteúdo existente nesta página é realmente notável para a experiência das pessoas,
Conhecem algum método para ajudar a evitar que o conteúdo seja roubado? Agradecia imenso.
Fiquei muito feliz em descobrir este site. Preciso de agradecer pelo vosso tempo
Também tenho o seu livro marcado para ver coisas novas no seu blog.
e dizer que gosto muito de ler os vossos blogues.
Obrigado|Olá a todos, os conteúdos existentes nesta
مرحبًا، أعتقد أن هذه مدونة ممتازة. لقد عثرت عليها بالصدفة ;
Kan du anbefale andre blogs / websteder / fora, der beskæftiger sig med de samme emner?
pokračujte v pěkné práci, kolegové.|Když máte tolik obsahu a článků, děláte to?
Tak Hej der til alle, det indhold, der findes på denne
webside er virkelig bemærkelsesværdig for folks oplevelse, godt,
že spousta z něj se objevuje na internetu bez mého souhlasu.
الاستمرار في توجيه الآخرين.|Ahoj, věřím, že je to vynikající blog. Narazil jsem na něj;
Esta página tem definitivamente toda a informação que eu queria sobre este assunto e não sabia a quem perguntar. Este é o meu primeiro comentário aqui, então eu só queria dar um rápido
) Znovu ho navštívím, protože jsem si ho poznamenal. Peníze a svoboda je nejlepší způsob, jak se změnit, ať jste bohatí a
nogensinde løbe ind i problemer med plagorisme eller krænkelse af ophavsretten? Mit websted har en masse unikt indhold, jeg har
Díky moc!|Hej, jeg synes, dette er en fremragende blog. Jeg snublede over det;
det. Denne side har bestemt alle de oplysninger, jeg ønskede om dette emne, og vidste ikke, hvem jeg skulle spørge. Dette er min 1. kommentar her, så jeg ville bare give en hurtig
že spousta z něj se objevuje na internetu bez mého souhlasu.
nenarazili jste někdy na problémy s plagorismem nebo porušováním autorských práv? Moje webové stránky mají spoustu unikátního obsahu, který jsem vytvořil.
muito dele está a aparecer em toda a Internet sem o meu acordo.
Obrigado|Olá a todos, os conteúdos existentes nesta
také jsem si vás poznamenal, abych se podíval na nové věci na vašem blogu.|Hej! Vadilo by vám, kdybych sdílel váš blog s mým facebookem.
It contains fastidious material.|I think the admin of this website is actually working hard in favor of his site,
webside er virkelig bemærkelsesværdig for folks oplevelse, godt,
har også bogmærket dig for at se på nye ting på din blog Hej! Har du noget imod, hvis jeg deler din blog med min facebook
Esta página tem definitivamente toda a informação que eu queria sobre este assunto e não sabia a quem perguntar. Este é o meu primeiro comentário aqui, então eu só queria dar um rápido
Com tanto conteúdo e artigos, vocês já se depararam com algum problema de plágio?
skupině? Je tu spousta lidí, o kterých si myslím, že by se opravdu
Does your blog have a contact page? I’m having problems locating it but, I’d like to send you an email. I’ve got some ideas for your blog you might be interested in hearing. Either way, great website and I look forward to seeing it improve over time.
for the reason that here every material is quality based
ocenili váš obsah. Dejte mi prosím vědět.
devido a esta maravilhosa leitura!!! O que é que eu acho?
After going over a few of the blog posts on your web page, I honestly appreciate your way of blogging. I bookmarked it to my bookmark website list and will be checking back soon. Please check out my web site too and let me know what you think. https://www.sheffieldstateuniversity.com/
ocenili váš obsah. Dejte mi prosím vědět.
Tak Hej der til alle, det indhold, der findes på denne
This is really interesting, You are a very skilled blogger. I’ve joined your rss feed and look forward to seeking more of your excellent post. Also, I’ve shared your site in my social networks!
|Tato stránka má rozhodně všechny informace, které jsem o tomto tématu chtěl a nevěděl jsem, koho se zeptat.|Dobrý den! Tohle je můj 1. komentář tady, takže jsem chtěl jen dát rychlý
Hmm it seems like your website ate my first comment (it was extremely long) so I guess I’ll just sum it up what I submitted and say, I’m thoroughly enjoying your blog. I as well am an aspiring blog blogger but I’m still new to everything. Do you have any tips for rookie blog writers? I’d really appreciate it.
Muito obrigado!}
pokračujte v pěkné práci, kolegové.|Když máte tolik obsahu a článků, děláte to?
information.|My family members every time say that I am killing my time here
It contains fastidious material.|I think the admin of this website is actually working hard in favor of his site,
It contains fastidious material.|I think the admin of this website is actually working hard in favor of his site,
har også bogmærket dig for at se på nye ting på din blog Hej! Har du noget imod, hvis jeg deler din blog med min facebook
fortsæt det gode arbejde stipendiater. Med at have så meget indhold og artikler gør du det
Com tanto conteúdo e artigos, vocês já se depararam com algum problema de plágio?
at web, except I know I am getting familiarity all the time by reading thes pleasant posts.|Fantastic post. I will also be handling some of these problems.|Hello, I think this is a great blog. I happened onto it;) I have bookmarked it and will check it out again. The best way to change is via wealth and independence. May you prosper and never stop mentoring others.|I was overjoyed to find this website. I must express my gratitude for your time because this was an amazing read! I thoroughly enjoyed reading it, and I’ve bookmarked your blog so I can check out fresh content in the future.|Hi there! If I shared your blog with my Facebook group, would that be okay? I believe there are a lot of people who would truly value your article.|منشور رائع. سأتعامل مع بعض هذه|
Podem recomendar outros blogues/sites/fóruns que tratem dos mesmos temas?
Hello! Someone in my Facebook group shared this site with us so I came to give it a look. I’m definitely enjoying the information. I’m book-marking and will be tweeting this to my followers! Fantastic blog and excellent style and design.
enten oprettet mig selv eller outsourcet, men det ser ud til
Wonderful blog! I found it while searching on Yahoo News. Do you have any suggestions on how to get listed in Yahoo News? I’ve been trying for a while but I never seem to get there! Thank you
på grund af denne vidunderlige læsning !!! Jeg kunne bestemt virkelig godt lide hver eneste lille smule af det, og jeg
Muito obrigado!}
fortsæt med at guide andre. Jeg var meget glad for at afdække dette websted. Jeg er nødt til at takke dig for din tid